How are Anti Iba1 antibodies used in the field of immunochemistry?

Immunocytochemistry (ICC) is a common laboratory method that uses antibodies to target specific peptides or protein antigens (i.e., antibody generators) in a cell by way of specific epitopes.

 

Epitopes are the part of the antigen that is recognized by the immune system (specifically by antibodies, B cells or T cells.) Once bound to the epitopes, the antibodies can then be detected using several different analytical methods.

 

ICC therefore allows researchers to evaluate whether or not cells in a particular sample display the antigen in question. In cases where an immunopositive signal is found, that is, the antigen is detected, ICC also allows researchers to determine in which sub-cellular compartments the antigen can be found.

The Western blot (sometimes called the protein immunoblot) is a widely accepted analytical technique used to detect specific proteins in a given sample. This technique uses gel electrophoresis to separate native proteins by 3-D structure, or denatured proteins by the length of the polypeptide.

 

The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specifically related to the targeted protein.

Iba1 (i.e., ionized calcium binding adaptor molecule 1) is a 17-kDa EF hand protein that is specifically expressed in microglia (the resident macrophages of the brain and spinal cord) and is upregulated during the activation of these cells.

 

Microglia act as the first and main form of active immune defense in the central nervous system. The Anti Iba1 antibody is specifically related to the Iba1 to which microglia react.

 

Anti Iba1 antibodies are appropriate for immunocytochemistry cell cultures, when used in combination with monoclonal antibodies that bind to GFAPs (Glial Fibrillary Acidic Proteins).

 

Anti Iba1 antibodies are also effective in the protein-staining procedure of the Western blot.

 

Wako Chemicals USA has developed rabbit polyclonal Anti IBA1 antibodies that have been formed against a synthetic peptide that corresponds to the Iba1 carboxy-terminal sequence. This is the sequence that is found in human, rat and mouse Iba1 protein sequences.

 

Wako’s Anti IBA1 antibodies are specifically related to microglia, but they are not cross-reactive with neurons and astrocytes (the most abundant types of cells in the brain and spinal cord.) Wako offers types of these antibodies respectively for immunocytochemistry and Western blot testing.

 

We are pleased to present Wako Chemicals USA, a provider of Anti IBA1 antibodies:

 

Wako Chemicals USA supplies clinical diagnostic reagents, laboratory chemicals, automated systems and workstations, and specialty chemicals of the highest quality, with a commitment to the public to contribute to the development of scientific technologies and the advancement of academic research.

 

Learn more about the Profile, Products, Address, Phone Number of Wako Chemicals USA.

 

Or, contact Wako Chemicals USA directly to learn more about their Anti IBA1 antibodies.